Composite

Part:BBa_K2328054:Design

Designed by: Yiyuan Gao   Group: iGEM17_TJU_China   (2017-10-20)


HU + Linker I + AcmA + Linker II + Linker.a + smURFP III + Histag.a + pMB1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 468
    Illegal PstI site found at 581
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 468
    Illegal NheI site found at 750
    Illegal PstI site found at 581
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 468
    Illegal BglII site found at 131
    Illegal XhoI site found at 125
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 468
    Illegal PstI site found at 581
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 468
    Illegal PstI site found at 581
    Illegal NgoMIV site found at 969
    Illegal NgoMIV site found at 1046
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2132


Design Notes

sho sakurai


Source

love

References